Journal: Veterinary Research

Article Title: Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors

doi: 10.1186/s13567-025-01548-2

Figure Lengend Snippet: Proposed model of T3SS effector actions delivered by the API1 injectisome in A. schubertii. During interaction with host cells, A. schubertii translocates several T3SS effectors into the host cytosol via the API1 injectosome. AopL induces caspase-3/-7-independent necrosis, possibly by targeting the lysosomal V-ATPase, a prediction supported by its homology with VopQ from Vibrio parahaemolyticus . In contrast, AopU promotes caspase-3/-7-dependent apoptosis. These cytotoxic effects are counteracted by AopI, a pro-survival effector, which, based on its structural similarity to P. aeruginosa ExoY, is presumed to act as a nucleotidyl cyclase. Sequence homology and domain analysis also suggest that AopU, AopH, and AopO induce cell rounding and inhibit phagocytosis, while AopI and AopJ may interfere with host immune signaling pathways. The specific role of AopT remains uncertain.

Article Snippet: For loading control, membranes were re-probed with mouse monoclonal IgG against β-actin (dilution 1:10 000; Proteintech, Cat. No. 66009-1-Ig) and revealed with secondary HRP-anti-mouse IgG (Cytiva), as above.

Techniques: Sequencing, Protein-Protein interactions